The present study succeeded in cultivating normal adult rat hepatocytes for at least 85 days without losing their replicative potential and differentiation capacity.
Ann N Y Acad Sci. 1980;349:85-98. Phenotypic stability of adult rat hepatocytes in primary monolayer culture. Bissell DM, Guzelian PS. PMID: 7013612.
We describe in detail the technique of hepatocyte isolation and establishment of primary cell cultures of adult rat hepatocytes. These cultures contain hormonally.
Long-term monolayer cultures of adult rat hepatocytes were tested for their ability to glucuronize phenol red and to maintain initial levels of cell proteins, glucose.